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Step 1- Diagnosis Blood Test for Vitamin B12: This test determines the level of cobalamin (B12) in the blood

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Deficiency in B12 can lead to a type of anemia called megaloblastic anemia, characterized by larger-than-normal red blood cells

Patients taking certain medications (e.g., chloramphenicol may reduce the effectiveness of B12 therapy) or with risk factors for B12 deficiency (metformin use, proton pump inhibitors, nitrous oxide exposure, vegan diet) should discuss these with their healthcare provider

Pull air into the syringe equal to the chosen water volume, inject that air into the BAC water vial, invert the vial, and slowly draw the exact volume